Table 6.
Thermal stability of VEGF G4 modification sequences at lower concentrations of cosolvents. 30% ACN increased thermal profiles of each modification as compared to their respective controls in 10 mM KCl. No marked changes in enthalpy were observed. Error bars are omitted for clarity and are <10% of the values shown; ND = not determinable.
| G41-4 | G4mod1 | G4mod2 | G4mod3 | |||||
|---|---|---|---|---|---|---|---|---|
| ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | |
| Control | 62 | 21 | 50 | 12 | ND | ND | 73 | 16 |
| 10 mM KCl | 67 | 29 | 68 | 29 | 68 | 27 | 67 | 25 |
| 10 mM KCl + 30% ACN | 83 | 33 | 80 | 24 | 84 | 24 | 80 | 20 |