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. 2018 May 16;2018:4597321. doi: 10.1155/2018/4597321

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Copyright © 2018 Elisa Borsani et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Quantitative evaluation of collagen type I (Col I) immunopositivity in osteoblasts (HOB) after 3 days of culture as IOD (Integrated Optical Density). A micrograph as an example has been reported. The cells were cultured in complete medium (OGM) or Concentrated Growth Factor (CGF), which represents the controls, and in presence of resveratrol 10 μM (R10), alendronate 5 μM (AL5), zoledronate 5 μM (ZOL5), alendronate 5 μM and resveratrol 10 μM (AL5 + R10), and zoledronate 5 μM and resveratrol 10 μM (ZOL5 + R10). Data represent means ± SE, ^∗p < 0,05 and in particular °p < 0,05 versus OGM; ^#p < 0,05 versus CGF; ^▴p < 0,05 versus OGM + R10; ^▪p < 0,05 versus CGF + R10.