Table 2.
Analytical parameters of system suitability tests for the proposed UPLC method.
| Parameter | Reference value | PAR∗ | TIZ∗ | ACF∗ | NIM∗ |
|---|---|---|---|---|---|
| Flow rate (mL/min) | — | 1.25 | |||
| Retention time (min) | — | 1.42 | 2.32 | 3.63 | 5.63 |
| Resolution (R) | R > 1.5 | — | 7.16 | 4.81 | 9.21 |
| K′ (column capacity) | >2 | 5.48 | 9.55 | 15.51 | 24.59 |
| Symmetry | — | 1.24 | 1.40 | 1.18 | 1.18 |
| Tailing factor (T) | ≤2 | 1.22 | 1.38 | 1.16 | 1.21 |
| N (column efficiency) | ≥2000 | 2979.69 | 3036.93 | 5090.82 | 6074.17 |
| HETP | =L/N | 0.033 | 0.032 | 0.019 | 0.016 |
∗Mean of three determinations.