Figure 4.

Percentage of viable cells—human keratinocytes (HaCat), after stimulation with M. officinalis leaves and stems extracts (20, 100, 250, 500, and 1000 μg/mL) for 24 h. The viability was assessed by using Alamar blue assay and was expressed as percentage of viable cells (%) related to positive control (cells stimulated with EtOH, the solvent used for obtaining the extracts). Data are expressed as mean values ± SD of three independent experiments done in triplicate and ^∗∗∗p < 0.001 and were calculated by one-way ANOVA followed by Tukey's post hoc test.