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(a) Male-specific k-mer counts above 30× coverage in Poecilia reticulata and P. wingei and shared across both; (b) female-specific k-mer counts above 30× coverage in P. reticulata and P. wingei and shared across both; (c) homology of assembled Y-linked contigs to each linkage group in the P. reticulata genome, showing the percentage of each linkage group covered by contigs ((bp length of contigs/bp length of linkage group) × 100).
