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In vitro characterization of selected TFs. (A) The translocation of IFN regulatory factor-1 (IRF1) and CREB1 to the nucleus was analyzed by Western blot in 3 independent experiments upon stimulation with pepsin-trypsin digested gliadin (PT-G) or pepsin-trypsin digested BSA (PT-BSA) as control; (B) Band intensity was quantified and normalized to the intensity of the band corresponding to HDAC1. Mean values were compared with a t-test. Data are expressed as mean ± SEM.
