Figure 1.

Proteins identified to associate with CHIKV nsP3 HVD. (A) The organization of CHIKV genome, the domains of nsP3 and schematics of constructs for expressing EGFP-CHIKVnsP3HVD and non-tagged EGFP in Flp-In T-REx cells; (B) Immunoblot analysis using anti-EGFP antibodies and immunoprecipitated (IP) samples obtained from the parental Flp-In T-Rex cells and transgenic T-REx-EGFP and T-REx-EGFP-CHIKVnsP3HVD cell lines; (C) Interaction network of proteins associated with the HVD of CHIKV nsP3 was created using STRING database; for this analysis proteins that showed at least 2-fold enrichment in SILAC-based quantitative assay were chosen.