Figure 4.

Effects of mutations in SH3 domain ligands on CHIKV RNA infectivity and CHIKV replication. (A) BHK-21 cells were electroporated with 1 μg of in vitro transcribed RNAs of CHIKV (wt or bearing mutation in nsP3 HVD); the infectivity of the transcripts was analysed by ICA. Mutant virus RNA infectivity is presented as a percentage of wild-type virus RNA, mean values together with standard error of three independent experiments are shown; (B) Growth curve of wt CHIKV or its mutant variants in BHK-21 cells infected at MOI of 0.1. Mean values of four replicates with standard deviation are shown. * denotes p < 0.05 (according to Student’s t-test), in comparison with wt virus.