Chemotactic migration of BL cells with latency III in response to CCL2. Mutu cl.99, Mutu III, and Jijoye P79 are BL cell lines with latency III; Mutu cl.148 and Jijoye M13 with latency I; and Mutu 30, the EBV-negative BL cell line. U937 (ATCC CRL 1593), the EBV-negative human histiocytic lymphoma cell line, was included as a positive control. For U937 cells, the assay was conducted using a membrane with 5-μm pores. (A) The results are shown as the relative migration (%): the values of migration into the MCP1-free chemotaxis medium were set as 100. The data are presented as the mean and the SD errors of duplicate samples in two independent experiments. (B) Significant differences in the migratory activities were defined in the pair-wise comparisons: Mutu cl.148 cells versus Mutu III (for concentrations 50 and 100 ng/mL), p < 0.001 (the top left panel); Mutu cl.148 cells versus Mutu cl.30 (for all four concentrations), p < 0.001 (the bottom left panel); Jijoye M13 versus Jijoye P79 (for all four concentrations), p < 0.001 (the right bottom panel). No significant differences were detected when comparing Mutu cl.148 and Mutu cl.99 at all concentrations, except one, the 200 ng/mL (the right top panel).