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. 2018 May 10;10(5):249. doi: 10.3390/v10050249

Figure 2.

Figure 2

TAGDD-1 does not affect initial steps of RSV replication. A549 cells were infected with RSV MOI 3 and treated with TAGDD-1 at 50 and 100 μM concentrations at 1 h after infection. The cells were harvested 24 h later to extract total RNA (A,B) or cellular protein (C). (A) Real-time PCR for RSV-N transcripts normalized to control RSV only using 2−ΔΔCt method (B) Absolute quantification by real-time PCR for genomic RSV (-) RNA transcripts normalized to control RSV only. (C). Western blot for RSV proteins. Total of 30 μg of protein was used per lane. The membrane was probed using rabbit polyclonal anti-whole RSV antibody (Bio-Rad, Hercules, CA, USA). The membrane was re-probed with anti-human β-actin antibody for loading control. (D) Light microscopy photograph (20×) of HEp-2 cells uninfected and infected with RSV MOI of 0.01 for 48 h in the presence of DMSO or TAGDD-1 at 50 μM concentration.