Table 1.

Oligonucleotides used to produce HSV-2 and HSV-1 UL21 gRNAs.

gRNAs	Predicted Nucleotide Cleavage Site a	Top Strand (5′-3′)	Bottom Strand (5′-3′)
HSV-2 UL21 gRNAs	nt52	5′-CACCGGACGTTGTGTTTTACGTCA-3′	5′-AAACTGACGTAAAACACAACGTCC-3′
	nt183	5′-CACCGACAGGCCCCAAAGACCGCA-3′	5′-AAACTGCGGTCTTTGGGGCCTGTC-3′
HSV-1 UL21 gRNAs	nt87	5′-CACCGGGCCTACTTTGTGTGCGGG-3′	5′-AAACCCCGCACACAAAGTAGGCCC-3′
	nt184	5′-CACCGACAGGCCCAGACGACCGCG-3′	5′-AAACCGCGGTCGTCTGGGCCTGTC-3′
	nt1540	5′-CACCGCCAGCCGCACGCGGGCCGC-3′	5′-AAACGCGGCCCGCGTGCGGCTGGC-3′

^a Nucleotide position in the UL21 gene targeted by the gRNA.