Figure 3.
Biodistribution of siRNAs in HBV transgenic mice. Representative fluorescence images obtained from samples harvested 10 min after injection of lipoplexes containing Alexa Fluor 750-labeled siRNAs. Three mice (i–iii) received intravenous injection of the uncomplexed naked labeled siRNA (A), and three mice (i–iii) received labeled siRNA within polyglutamate-containing lipoplexes (B). One mouse, which received a saline injection, served as the negative control (C). Fluorescence detectable in livers (Li), lungs (Lu), kidneys (Ki), and spleens (S) are shown in the top row of images. Microscopy of frozen sections from liver and kidney samples are also shown below. The scale bar indicates 50 μm. Quantitation of fluorescence, radiant efficiency, was measured in the organs of mice given naked guanidinopropyl (GP3)-siRNA3 or lipoplexed GP3-siRNA3 (D). Data are represented as the mean radiant efficiency (±SEM) for each organ from the three animals (reproduced with permission from [88], © 2015 Elsevier B.V.).