Table 6.
Adoptive transfer (AT) Studies to test the role of immune T cells from prototype IWV-vaccinated cats a.
| Study/Group (Reference) |
AT Donor | Number of AT Recipient b | Type of MHC Matching c | AT Cell Type d | Challenge FIV (CID50) e | P-C Protect Rate [A vs. B, p-Value] f,g | Com Protect Rate [A vs. B, p-Value] f,g |
|---|---|---|---|---|---|---|---|
| Study 1 [68] | |||||||
| 1A | Vaccinated | 8 | Half-match | Blood cells | FIV-Pet (20) | 6/8 h [0.0070] | 6/8 g [0.0023] |
| 1B1 | Vaccinated | 2 | Unrelated | Blood cells | FIV-Pet (20) | 0/2 [1.0000] | 0/9 |
| 1B2 | Non-vaccinated | 7 | Half-match or Unrelated | Blood cells | FIV-Pet (20) | 0/7 | - |
| Study 2 [68] | |||||||
| 2A | Vaccinated | 3 | Half-match | Blood cells | FIV-Pet (50) | 2/3 i [0.0667] | 2/3 g [0.3000] |
| 2B1 | Vaccinated | 2 | Unrelated | Blood cells | FIV-Pet (50) | 0/2 | 0/7 |
| 2B2 | Non-vaccinated | 5 | Half-match or Unrelated | Blood cells | FIV-Pet (50) | 0/5 | - |
| Study 3 [68] | |||||||
| 3A | Vaccinated | 2 | Identical | Blood cells | FIV-Pet (100) | 2/2 | 2/2 [0.0667] |
| 3B | None | (4 Control cats) | NA | Blood cells | FIV-Pet (100) | 0/4 | 0/4 |
| Combined Partial-Complete Protection Vaccine Groups 1A + 2A + 3A: Combined Complete-Protection Vaccine Groups 1A + 2A + 3A: Combined Control Groups 1B + 2B + 3B: |
10/13 [<0.0001] | ||||||
| 6/13 [0.0013] 0/21 | |||||||
| Study 4 [62] | |||||||
| 4A | Vaccinated | 4 | MLR-match | T cells | FIV-Pet (25) | 3/4 | 3/4 [0.1429] |
| 4B1 | Vaccinated | 2 | MLR-match | B cells | FIV-Pet (25) | 0/2 | 0/4 |
| 4B2 | Non-vaccinated | 2 | Unrelated | PBMC | FIV-Pet (25) | 0/2 | - |
| Study 5 [62] | |||||||
| 5A1 | Vaccinated | 4 | Complete-match | T cells | FIV-Pet (25) | 4/4 [0.0286] | 8/10 [0.0419] |
| 5A2 | Vaccinated | 3 | Partial or Complete-match | CD4+ T cells | FIV-Pet (25) | 2/3 [0.1429] | - |
| 5A3 | Vaccinated | 3 | Partial or Complete-match | CD8+ T cells | FIV-Pet (25) | 2/3 [0.1429] | - |
| 5B1 | Non-vaccinated | 1 | MLR-match | T cells | FIV-Pet (25) | 0/1 | 0/4 |
| 5B2 | Non-vaccinated | 1 | Unrelated | PBMC | FIV-Pet (25) | 0/1 | - |
| 5B3 | None | (2 Control cats) | NA | PBS | FIV-Pet (25) | 0/2 | - |
| Study 6 [62] | |||||||
| 6A1 | Vaccinated | 5 | Complete-match | T cells | FIV-Pet (25) | 5/5 [0.0079] | 6/7 [0.0699] |
| 6A2 | Vaccinated | 2 | Partial-match | T cells | FIV-Pet (25) | 1/2 [0.3333] | - |
| 6B1 | Non-vaccinated | 1 | Unrelated | T cells | FIV-Pet (25) | 0/1 | 0/4 |
| 6B2 | None | (3 Control cats) | NA | PBS | FIV-Pet (25) | 0/3 | - |
| Combined Complete Protection Vaccine Groups 5A1 + 6A1: Combined Control Groups 5B1 + 5B2 + 5B3 + 6B1 + 6B2: |
9/9 [0.0001] 0/8 |
||||||
| Study 7 [62] | |||||||
| 7A1 | Vaccinated | 9 | Complete-match | T cells | FIV-FC1 (25) | 6/9 [0.009] | 6/12 [0.0419] |
| 7A2 | Vaccinated | 3 | Partial-match | T cells | FIV-FC1 (25) | 0/3 | - |
| 7B1 | Non-vaccinated | 3 | Unrelated | T cells | FIV-FC1 (25) | 0/3 | 0/8 |
| 7B2 | None | 5 | NA | PBS or None | FIV-FC1 (25) | 0/5 | - |
a Studies 1–3 and Studies 4–7 in Table 6 adapted and summarized from references [62,68], respectively. b Number of control cats (# Control cats) were without AT. c Half-match between parent to offspring; mixed leukocyte reaction (MLR) for MHC matching is a functional assay where more proliferation when MHC not matched; not applicable (NA). d PBS washed whole blood cells (Blood cells). e All studies with intravenous challenge at mean cat infectious dose (CID50). f Partial-Complete Protection (P-C Protect); Combined Protection (Com Protect); Studies A versus B [A vs. B]; two-tailed Fisher Exact Test for p-value (italics) with p < 0.05 as significant (bolded italics). g Only partial-complete protection combined for Studies 1 and 2; completed protection in Studies 3–6. h 3/8 complete protection and 3/8 partial protection; p-value comparing 1A vs. 1B2. i 1/3 complete protection and 1/3 partial protection; p-value comparing 2A vs. 2B2.