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. 2018 Apr 24;8(5):270. doi: 10.3390/nano8050270

Figure 3.

Figure 3

(A) Percentage of transfected A549 cells after the treatment by lipoplexes at different N/P ratios at siRNA concentration of 40 nM (4 h). Cells were post-treated with Trypan Blue solution; (B) Mean Fluorescence Intensity (MFI) was determined by flow cytometry 4 h after the treatment (n = 3). (C) Silencing efficiency of a splicing factor mRNA with lipoplexes carrying the corresponding siRNA targeting this splicing factor (siACT) or a siRNA control (siCR). A549 cells were transfected during 48 h by lipoplexes with 40nM of siRNA (n = 4). The calcium phosphate was used as a transfection positive control following the same procedure. One-way ANOVA, post test Dunnett’s, p < 0.1 (*), p < 0.01 (**) and p < 0.001 (***) compared to untreated cells (Blank); (D) Silencing efficiency of the targeted splicing factor protein with selected lipoplexes carrying the corresponding siRNA directed against this splicing factor (siACT) or a siRNA control (siCR). A549 cells were transfected during 72 h by lipoplexes with 40 nM of siRNA. The calcium phosphate was used as a transfection positive control (+). β-Actin was used as loading control.