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. 2018 May 30;38(22):5122–5139. doi: 10.1523/JNEUROSCI.3652-17.2018

Figure 1.

Figure 1.

Glycogen and glial glucose transporters decreased with glaucoma pathology. A, Glycogen analysis in 3-, 6-, and 10-month-old D2 and control D2G ONs (n = 6 ONs/group). B, F, Capillary electrophoresis of GLUT1 (B) and GLUT3 (F) protein in 3-, 6-, and 10-month-old D2 and D2G ONs normalized to total protein then to 3-month-old D2G protein levels (n = 8 ONs/group). See Figure 1-1. C, Distribution of GLUT1 in ONs of 3-month-old D2 and 10-month-old D2G and D2 optic nerves immunolabeled with GFAP (green). Arrows indicate colocalization of GLUT1 and GFAP (n = 3 sections/ON, n = 6 ONs/group). D, E, Glut1 and Glut3 mRNA levels in 3-, 6-, and 10-month-old D2 and D2G ONs, normalized to Hprt mRNA then to 3-month-old D2G mRNA (n = 5–7 ONs/group). See Figure 1-1. G, Distribution of GLUT3 in ONs stained with FluoroMyelin. Arrows indicate colocalization of GLUT3 with FluoroMyelin (n = 3 sections/ON, n = 6 ONs/group). H, I, Percentage of mean fluorescence intensity in the ROI for GLUT1 (C) and GLUT3 (G). All values are presented as the mean ± SEM, using one-way ANOVA and Tukey's post hoc test. A, F(5,30) = 18.59, **p = 0.0022, ***p = 0.00011; B, F(5,42) = 6.463, **p = 0.0077; D, F(5,30) = 1.906, **p = 0.001; E, F(5,37) = 15.58, **p = 0.0023, **p = 0.001; H, F(2,51) = 210.4, ***p = 0.0001. Scale bar, C, G, 20 μm.