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. 2018 May 30;38(22):5122–5139. doi: 10.1523/JNEUROSCI.3652-17.2018

Figure 2.

Figure 2.

Monocarboxylate transporters downregulated with glaucoma pathology. A, Mct1 mRNA levels in 3-, 6-, and 10-month-old D2 and D2G ONs, normalized to Hprt mRNA then to 3-month-old D2G mRNA (n = 5–13 ONs/group). See Figure 2-1. B, D, E, MCT1, MCT2, and MCT4 protein levels in 3-, 6-, and 10-month-old D2G and D2 ONs, normalized to total protein and then to 3-month-old D2G protein levels (n = 8 ONs/group). C, F, G, Distribution of MCT1 (C), MCT2 (F), and MCT4 (G) in 3-month-old D2 and 10-month-old D2G and D2 ONs stained with FluoroMyelin (green) or immunolabeled with GFAP (green). Arrows indicate colocalization (n = 3 sections/ON, n = 6 ONs/group). H, Distribution of MCT2 in human control and glaucoma patient ONs, immunolabeled for β-tubulin (green) and stained with DAPI (blue). Arrows indicate colocalization (n = 4 sections/ON, n = 2 ONs/group). See Figure 2-1. I–L, Percentage of mean fluorescence intensity in the ROI I, MCT1; J, MCT2; K, MCT4 (D2 and D2G mouse ONs); and L, MCT2, human ON; and (L) MCT2, human ON; and MCT2, human ONs (L). M, Quantification of the total number of RGC axons in 6-month-old D2, 10-month-old D2G, and 10-month-old D2 ONs. All values are presented as the mean ± SEM, one-way ANOVA and Tukey's post hoc test. A, F(5,47) = 4.579, *p = 0.0141; B, F(5,38) = 6.553, **p = 0.0076; D, F(5,42) = 16.14, *p = 0.0054, **p = 0.0013; E, F(5,42) = 4.734, *p = 0.0348; I, F(2,51) = 173.2, ***p = 0.0001; J, F(2,51) = 402.1, ***p = 0.0001; K, F(2,51) = 192.6, ***p = 0.0001; L, t(6) = 9.317, ***p = 0.0001, two-tailed unpaired t test. Scale bars, C, F–H, 20 μm.