Fig. 4.

Pin1 knockdown suppresses tumor growth of human leukemia cells in vivo. After treating HL-60 and U937 Tet-On cells with or without Dox (1 mg/ml) for 3 days in vitro, cells were subcutaneously implanted into the flanks of nude mice fed with a normal or a doxycycline-containing diet,respectively. a, b Tumor volumes were measured and the tumor growth curves were plotted over time. Error bars represent standard deviations. c, d Photographic illustration of tumors harvested from nude mice at the end point. Each scale of the ruler represents 1 mm. e, f Weights of tumors harvested from nude mice at the end point. Error bar represents SEM. g, h Total proteins from xenograft tumor samples were subjected to western blot analysis of the indicated proteins. Statistically significant differences using Student’s t test are indicated by p values. (*p < 0.05, **p < 0.01, ***p < 0.001)