Table 4.

Substitution saturation analysis of the CRA region based on the index of substitution saturation as implemented in DAMBE6

Marker dataset	Number of OTUa	Issb	Iss.cSymc	dfd	p valuee	Iss.cAsymf	dfd	p valuee
CRA-All	4	0.240	0.789	366	< 0.0001	0.757	366	< 0.0001
	8	0.259	0.743	366	< 0.0001	0.631	366	< 0.0001
	16	0.242	0.703	366	< 0.0001	0.494	366	< 0.0001
	32	0.260	0.692	366	< 0.0001	0.363	366	0.0001

^aNumber of sequences used in the random resampling

^bindex of substitution saturation

^ccritical value for a symmetrical tree topology

^ddegrees of freedom

^eprobability that I_ss is significantly different from the critical value (I_ss.cSym/I_ss.cAsym)

^fcritical value for an asymmetrical tree topology

Note: two-tailed tests are used