Figure 5.

Microarray analysis. Human gene filter arrays containing 5184 noncontrol cDNAs were hybridized with control (A) and 4-h peroxide-treated (B) samples derived from HA-1 cells extracted as described. cDNA was prepared from 6 μg of RNA in the presence of ³³P radiolabel and used to probe two separate human gene filter arrays. The figure images are representative array hybridization signals obtained following washing 2 × 20 min with 2× SSC plus 1% SDS at 50°C, then 0.5× SSC plus 1% SDS at 50°C for 30 min and phosphoimaging.