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. 2018 May 31;10(4):179–191. doi: 10.3727/000000002783992442

Figure 1.

Figure 1

Example of microarray analysis. Human gene filter arrays containing 5184 noncontrol cDNAs were hybridized with probes obtained from HL-60 control and HP100-resistant cells. The cDNA probes were prepared from 6 μg of extracted total RNA in the presence of 33P radiolabel. After hybridization and washing, a final wash stringency of 55°C and 0.5× SSC, 1% SDS was employed. The arrays were then phosphoimaged using a Storm 860 phosphoimager with ImageQuant software.