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Ephrin-As and EphA4 are expressed by neural crest cells, using RT-PCR. Ephrin-A2, ephrin-A5, ephrin-A6, and EphA4 transcripts were all detected in RNA samples from stage 15 whole embryos (whole) and samples from isolated migratory neural crest cells (NC). Wnt-3a, a marker for dorsal neural tube, was used as a control for neural tube contamination of neural crest RNA. β-Actin was used as an internal control.
