Fig. 5.

Antiapoptotic effect of miR-10a attenuated by restoration of KLF4. Lentivirus which carries KLF4 vector used to infect miR-10a-upregulated old hBM-MSCs (O-10a) to restore KLF4 expression (O-10a-KLF4). miR-10a-upregulated old hBM-MSCs (O-10a) also infected by the control lentivirus (O-10a-c). Cells cultured for 72 h under hypoxia conditions. a Cell apoptosis assayed by TUNEL staining. Percentage of apoptotic cells (TUNEL⁺) quantified in O-10a and O-10a-KLF4 hBM-MSCs. b Cell survival evaluated in O-10a and O-10a-KLF4 hBM-MSCs. c Protein expression of MCL1 and PUMA evaluated by western blot analysis in O-10a and O-10a-KLF4 hBM-MSCs. d Ratio of Bax/BCL2 quantified in O-10a and O-10a-KLF4 hBM-MSCs. e Protein expression of cleaved caspase-3 and inhibitor of caspase-activated DNase (ICAD) assayed in O-10a and O-10a-KLF4 hBM-MSCs. f Caspase-3 activity evaluated in O-10a and O-10a-KLF4 hBM-MSCs. n = 6/group. Mean ± SD. *P < 0.05. DAPI 4′,6-diamidino-2-phenylindole, KLF4 Krüpple-like factor 4, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling, RFU relative fluorescence units