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. 2018 May 30;18:12. doi: 10.1186/s12861-018-0171-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Diagram of distal C. elegans germline and experimental workflow. a The syncytial distal progenitor zone (highlighted in red based on WAPL-1 antibody staining) contains mitotically cycling stem and progenitor cells and cells in meiotic S-phase. The distal tip cell (DTC) provides GLP-1 signal (Notch ligand) to maintain the stem cell fate of these cells. As cells migrate away from the DTC, they exit the progenitor zone and enter meiotic prophase. b Workflow used to assay the relationship between PCN-1 accumulation and S-phase (EdU labeling) in the C. elegans germline