Figure 3.

miRNA-mediated gene signal transduction network. (A) Two functional modules in the miRNA-mediated gene signal transduction network. The miRNA-mediated gene signal transduction network was divided into two functional modules containing 19 and 3 nodes, respectively. The first module was significantly associated with PI3K-Akt signaling pathway (P = 0.02) and GTPase mediated signal transduction (P = 0.003), and the second one was involved into Insulin-like growth factor receptor signaling pathway (P = 0.02), which can potentially regulate the major pathological changes during RA progression. (B) Upstream differentially expressed miRNAs that regulate the interactions of MX1-OASL and RNF2-UST. Red and blue square nodes respectively refer to the upregulated and downregulated miRNAs in the responder group compared to the non-responder group. Red and blue circle nodes respectively refer to the upregulated and downregulated mRNAs in the responder group compared to the non-responder group. Purple edges refer to the gene-gene interaction with the highest edge-betweenness, suggesting their crucial roles in transferring signals. miRNA nodes with yellow marks refer to the most differentially expressed miRNAs that regulate MX1, OASL, RNF2, and UST, respectively.