Skip to main content
. Author manuscript; available in PMC: 2018 Jun 7.
Published in final edited form as: J Am Chem Soc. 2017 May 24;139(22):7533–7539. doi: 10.1021/jacs.7b01599

Figure 5.

Figure 5

(a) Chemical structure of macrocyclic heptaoxazole (7OTD) and its fluorescence derivative Cy5–7OTD (a Cy5 fluorophore linked to 7OTD). (b) Visualization of the telomere RNA G-quadruplex formed by 24-mer RNA with Cy5–7OTD. RNA bands were stained with GelStar (green). Complexes of the G-quadruplex and Cy5–7OTD were detected by the fluorescence of Cy5–7OTD (red). Concentrations of Cy5–7OTD are shown above the gel. (c) Visualization of ds-RNA and ss-RNA with Cy5–7OTD. RNA bands were stained with GelStar (green). Concentrations of Cy–7OTD are shown above the gel. Conditions: 20% PAGE in 1× TBE buffer with 20 mM NaCl at 4 °C for 8 h (80 V).