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. Author manuscript; available in PMC: 2018 May 31.
Published in final edited form as: Cancer Res. 2011 Sep 12;71(21):6665–6675. doi: 10.1158/0008-5472.CAN-11-1590

Figure 2. Vascular Invasion and Metastasis in Kras-Tgfbr2−/− Mice.

Figure 2

Kras-Tgfbr2−/− tumor cells (Panel A, B) that are KRT18 positive (inset Panel B) invade through the vessel wall into intima with an endothelial cell layer overlying the nest. This is confirmed by CD31 immunohistochemistry in endothelium (arrows) in Panel C over the non-staining tumor cells (asterisk). A small fibrin thrombus is seen between the arrows. Visceral pleural invasion is seen using elastic stain (Panel D) demonstrating intact pleural elastic tissue (left arrowhead) and tumor cell invasion through the visceral pleural elastic tissue, causing a defect (right 2 arrowheads) and tumor growth on the pleural surface. Discrete foci of tumor cells were detected within mediastinal lymph nodes (E, F). The epithelial origin of these cells was confirmed by KRT18 immunostaining (inset F). (Hematoxylin and eosin, Original magnification × 50, A, E; and × 150 B, F; Inset B and F DAB IHC Original magnification × 150; C, DAB IHC Original magnification × 150; D, Elastic von Gieson, Original magnification × 25).