Table 3.

Editing by ADAR1 and K744 of dsRNA substrates with a 31-bp stem

	E1 E2 * *		ADAR1	K744
RG1E	5′ uCA UUA aGG UgG GUG GaA UGC UAU AAC AAu AUg cuca	E1	48 (8)	22 (16)
	3′ uGU AGU cCC AuC CAC CcU ACG AUA UUG UUg UA_____,	E2	73 (3)	47 (11)
RG2B	5′ uCA UUA aGG UgG GUG GaA UGC AUA UAC AAu AUg cuca	E1	47 (3)	ND
	3′ uGU AGU cCC AuC CAC CcU ACG UAU AUG UUg UA_____,	E2	74 (3)	ND
RG3A	5′ uCA UUA aGG UgG GUG GaA UGC GCG CGC AAu AUg cuca	E1	49 (6)	25 (20)
	3′ uGU AGU cCC AuC CAC CcU ACG CGC GCG UUg UA_____,	E2	69 (7)	42 (13)
RG10A	5′ uCA UUA aGG UgG GUG GaG CGC GCG CAU AAu AUg cuca	E1	59 (12)	26 (9)
	3′ uGU AGU cCC AuC CAC CcC GCG CGC GUA UUg UA_____,	E2	74 (3)	52 (11)
RG15D	5′ uCA UUA aGG UgG GUG GCG CGC GCU AAC AAu AUg cuca	E1	43 (1)	28 (6)
	3′ uGU AGU cCC AuC CAC CGC GCG CGA UUG UUg UA_____,	E2	66 (7)	53 (12)

Z-forming substrate sequences do not enhance editing either by ADAR1 or the deletion mutant K744. A variety of editing substrates were tested, some of which contain alternating purine and pyrimidine sequences that are favored to form Z-DNA/Z-RNA. The two adenosines that are edited are asterisked and highlighted in bold in the middle column. The one on the left is referred to as E1, and the one on the right as E2, with E2 corresponding to the adenosine deaminated in the RG substrate in vivo. Quantitation for each substrate is given in the right-hand column, with the upper value corresponding to E1 and the lower value to E2. Quantitation is based on three experiments, with the standard deviation shown in parentheses.