Figure 8.

Application of SiNW FET in drug discovery. (a) The binding of negatively charged ATP to tyrosine kinase enzyme activates the phosphorylation of tyrosine protein and release ADP. In the presence of uncharged Gleevec molecules, the immobilized tyrosine kinase on the SiNW surfaces is inhibited by the competitive binding of the Gleevec molecules. (b) Real-time monitoring of ATP binding to the immobilized tyrosine kinase in the presence of different concentration of Gleevec molecules. The ATP concentration is fixed at 240 nM. (c) Conductance change (ΔG) vs. ATP concentration for Abl-functionalized SiNW in the presence of different concentrations of Gleevec: red (0 nM), green (1 nM), and blue (3 nM). Reprinted with permission from Ref. [65]. Copyright (2005), PNAS.