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. 2018 Apr 27;9(32):22586–22604. doi: 10.18632/oncotarget.25187

Figure 4. LRP5 regulates STK40 expression at the transcriptomic level.

Figure 4

(A-C) HCC38 cells were transfected with one of two different siRNAs against LRP5 (#2: red square; #4, red triangle) or LRP6 (#7: blue square; #8, blue triangle) or with a control siRNA (gray circle). This experiment was performed in duplicate. Expression profiling was performed with Gene 2.1 Affymetrix chips, for HCC38 cells 24 h, 48 h and 72 h after transfection. (A-B) The expression of LRP5 (A) or LRP6 (B) was first checked in LRP5- and LRP6-depleted cells, to validate the experiment. (C) Microarray analysis revealed that STK40 RNA levels were lower specifically in LRP5-depleted HCC38 cells. (D) We confirmed, by RT-qPCR, that STK40 RNA levels were specifically lower in HCC38 cells depleted of LRP5. The data shown are means ± SD from three independent experiments. *P<0.05, **P<0.01, ***P<0.001 (i.e. a decrease relative to the control siRN A).