Figure 3. ALDH3A1 knock-down reduces cell viability and sensitizes cells to TMZ.

(A) Two individual knock-downs of ALDH3A1 were established in three cell lines. α-Tubulin served as gel loading control. (B) ALDH3A1 knock-down (crALDH3A1.2) and control cells were treated with two different concentrations of TMZ, based on the IC₅₀ of the control cells (1 × IC₅₀, 0.5 × IC₅₀). Cell viability was measured at day 0, 4, 6 (and 8 in slowly growing cells). Statistical evaluation was performed for ALDH3A1 knock-down cells compared to control cells treated with DMSO or the same concentration of TMZ. (C) Cell viability of the DMSO-treated cells of either ALDH3A1 knock-down or control cells is normalized to 100%. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 (unpaired student t-test). D: DMSO.