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. 2018 Apr 27;9(32):22785–22801. doi: 10.18632/oncotarget.25237

Figure 2. Analysis of CD157 expression in vesicles released by mesothelial cells.

Figure 2

Western blot analysis of CD157 expressed in total cell lysate (30 μg/lane), cell culture medium (1 ml, TCA precipitated) and vesicles (30 μg/lane) from (A) non-malignant Met-5A mesothelial cells or (B) CG98 mesothelioma cells, detected by SY/11B5 mAb. A representative experiment is shown (n = 3). (C) Western blot analysis of CD157 and CD81 expression in exosomes obtained from serum-free culture medium of CG98 cells by sucrose density gradient fractionation. Twelve fractions were collected from the top of the gradient, proteins from each fraction were precipitated with methanol/chloroform, separated by 10% SDS-PAGE, transferred to PVDF membranes and immunoblotted with anti-CD157 or anti-CD81 mAb. A representative experiment is shown (n = 3).