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. 2018 May 24;6:66. doi: 10.3389/fbioe.2018.00066

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Copyright © 2018 Belgodere, King, Bursavich, Burow, Martin and Jung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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An example of 3DBP breast cancer microenvironments. (A) Schematic diagram of direct, 3D bioprinted, cell-laden bone matrix as a biomimetic model for a breast cancer metastasis study. (B) CAD model of the 3D matrix. (C) 3D surface plot of the bioprinted matrix. Scale bar: 200 μm. (D–G) Scanning electron micrographs (cross-sectional view) of porous matrices: (D) 10% GelMA, (E) 10% GelMA + nHA, (F) 15% GelMA, and (G) 15% GelMA+nHA, respectively. Scale bar: 100 μm. The inset images are photographs of the corresponding matrices. (H,I) Fluorescence micrographs of the 3D bioprinted MSC-laden 10% GelMA matrix; 3D bioprinted cells were prelabeled by Cell Tracker Green CMFDA dye. GelMA; gelatin methacrylate (A–I reproduced with permission from Zhou et al., 2016).