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. 2018 Apr 12;37(10):e96941. doi: 10.15252/embj.201796941

Figure 5. Reversible transitions of the HNH positioning in the ternary complex.

Figure 5

  1. Representative time trajectory of the FRET efficiency, showing the fluctuation of the HNH domain in the sgRNA/DNA‐bound S355C–S867C complex with Mg2+. The transition points of the FRET efficiency (blue line) were detected using the HMM algorithm (black line).
  2. The transition density plot of different FRET states of the sgRNA/DNA‐bound S355C–S867C complex with Mg2+. The density map was clustered into five groups (white, gray, black, green, and blue closed circles) based on the k‐means method with k = 5, suggesting that the HNH movement between the D* and D processes (middle and high FRET efficiencies) is rare.
  3. Bar plot of the dwell times for each transition. The mean dwell times were determined by fitting the dwell time distributions (n = 399, 223, and 136 for low, middle, and high FRET status, respectively) to a single exponential decay function (Appendix Fig S2). Error bars show SEM.

Source data are available online for this figure.