Figure 2.

Validation of the tissue specificity of the putative nonendosperm tissue‐expressed promoters in transgenic rice. Histochemical staining was conducted in a series of tissues from transgenic plants including the roots, leaves, stems and sheaths of 60 DAG rice plants and the panicles of 75 DAG plants (a), anthesis and seeds at different developmental stages (b). The seeds were cut into median longitudinal sections. In transgenic plants carrying the construct of the tissue‐expressed promoters, strong blue staining was observed in various nonendosperm tissues after only 2 to 12 h of incubation, while no GUS staining was detected in endosperms after 24 h of X‐gluc incubation. Scale bars represent 2 mm.