| Overall objective/ Purpose |
“Development of a method to isolate small RNAs from different plant species (...) that no need of first total RNA extraction and is not based on the commercially available TRIzol® Reagent or columns.” |
Protocol available at Rosas-Cárdenas et al. (2011)
|
| Application |
“DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping.” |
Protocol available at Lu (2011)
|
| Advantage(s) |
“We describe a fast, efficient and economic in-house protocol for plasmid preparation using glass syringe filters. Plasmid yield and quality as determined by enzyme digestion and transfection efficiency were equivalent to the expensive commercial kits. Importantly, the time required for purification was much less than that required using a commercial kit.” |
Protocol available at Kim & Morrison (2009)
|
| Limitation(s) |
“A major problem faced both in this and other safflower transformation studies is the hyperhydration of transgenic shoots which result in the loss of a large proportion of transgenic shoots.” |
Protocol available at Belide et al. (2011)
|
