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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 14;200(8):2957–2964. doi: 10.4049/jimmunol.1800022

Figure 3. Human antibodies specific for class II SLA can initiate complement mediated cytotoxicity.

Figure 3

7 sera lacking class II HLA antibodies and 7 sera containing class II HLA antibodies were incubated with cells expressing pig CD74 and individual combinations of class II SLA alleles. Table 2 describes the class II allele combinations. Parent cells were HEK 293T cells devoid of CD74 and class II SLA. Rabbit complement was added and dead cells counted as an indicator of antibody initiated complement cytotoxicity (percent killing). (A) Cytotoxicity of sera lacking class II HLA antibodies. (B) Cytotoxicity of sera containing class II HLA antibodies. DTT was added to inactivate IgM in panels C (sera lacking class II HLA antibodies) and D (sera containing class II HLA antibodies). DR indicates SLA-DRα/DRβ pairs, DQ represents SLA-DQα/DQβ pairs, and H represent SLA-DRα/DQβ pairs. Parent represents killing against cells lacking both class II SLA proteins and CD74. Panel E shows the reduction of cell killing for a single serum against cells expressing all 10 class II SLA proteins before and after DTT treatment (±DTT comparison p = 0.001, Wilcoxon test).