Figure 6. Nanopattern Fabrication with LSL.

A 10 nm Au surface was functionalized with an OEG-terminated alkanethiol and a mixed array of 1×1 and 1×3 pixel ROIs was used to thermally desorb the OEG SAM with a 532 nm laser focused through a 63× (NA1.4) oil immersion objective operating at a constant fluence of 33.38 nJ μm⁻² with iterations varying from 125–10,000 per ROI. The surfaces were exposed to HFN, the HFN fluorescently labeled, and the surfaces imaged. Fluorescently labeled HFN adsorbed to the patterned array created with (A) 125 iterations or (B) 10,000 iterations per ROI. The (C) diameter and (D) area of the circular patterns increased with increased iterations due to spreading of the heat through the Au films. (A,C) Patterns as small as 450 nm (0.17 μm²) were created using LSL. SB=5μm.