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. 2018 Apr 9;37(10):e96553. doi: 10.15252/embj.201796553

Figure 8. TLR9 mediates inflammation upon Opa1 deficiency.

Figure 8

  • A
    Quantification of TLR9 expression immunostaining in gastrocnemius muscle of Opa1 KO animals at 3, 7, 10, 13, and 20 weeks of age. This quantification was performed using Trainable Weka Segmentation plugin from ImageJ (five images per group). Represented values are relative to loxP group.
  • B–E
    MyD88, S100A8, HMGB1, and RAGE expression in Scr and KD myoblasts treated with ODN2088 (ODN) or ODN2088 negative control (C) 1 μM (n = 9).
  • F
    IL‐6 levels detected in cultured medium of myoblasts (Scr and KD) treated with ODN2088 (ODN) or ODN2088 negative control (C) (n = 9).
  • G, H
    Expression of genes of TLR9 (G) and Opa1 (H) upon single or double knock‐down. n = 8 (G); n = 3 (H).
  • I
    Expression of NF‐κB target genes in control (Scr), Tlr9 depleted (Tlr9 KD), Opa1 depleted (Opa1 KD), and double Tlr9‐Opa1 ablated (DKD) cells (n = 3).
Data information: Data represent mean ± SEM. *P < 0.05 when comparing the effect of Opa1 deficiency. # P < 0.05 when comparing the effects of ODN2088 (in panels B–F) or comparing to Opa1 KD cells (in panels G–I). Data were analyzed using analysis of variance with Tukey's post hoc test.