Figure 6.

Inhibition of alloantigen-induced T cell response in a draining lymph node by compounds 6, 15, 18, and 19. Mice (Balb/c) received a footpad injection of allogeneic splenocytes (isolated from DBA-2 mice) and were treated with anti-CD154 antibody (MR-1; 20 mg/kg, day −1 and 0) or compounds as shown (10–40 mg/kg, b.i.d. from day −1 to 3, s.c. in 20% HPβCD). Draining (popliteal) lymph nodes were collected three days after the alloantigen challenge and cells were counted. Data are shown as mean ± SD. Significant differences compared to vehicle-treated controls are indicated by asterisks (**** p < 0.0001; one-way ANOVA followed by Dunnett’s multiple comparison test).