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Kinetic parameters for the CMP-Neu5Ac hydrolysis activities of Pd2,6ST and Pd2,6ST S232L/T356S/W361F neosialidase.
| Enzymes and mutants | kcat(min−1) | KM(mM) |
kcat/KM (min−1mM−1) |
|---|---|---|---|
| Pd2,6ST[a] | (4.0±0.9)×102 | 45±14 | 8.8 |
| Pd2,6ST S232L/T356S/W361F | (1.1±0.01)×104 | 3.7±0.1 | 3.0 × 103 |
Reported previously.35
