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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Dev Biol. 2018 Apr 22;439(2):92–101. doi: 10.1016/j.ydbio.2018.04.015

Figure 3. Enterocyte maturation is particularly susceptible to TFAM-loss.

Figure 3

(A) Alkaline phosphatase activity is diminished in the duodenum of TFAMKO embryos compared to littermate controls at E18.5, whereas goblet cells appear unaffected (B). (C) Proliferation in the intervillus regions appears similar between control and TFAM-mutant embryos at E18.5. (D) qRT-PCR analysis of transcripts isolated from the E18.5 whole gut epithelium indicate that markers of enterocyte or proliferating cells are expressed in a manner consistent with histological analysis. These data point to a specific requirement for TFAM in enterocyte maturation, whereas TFAM function appears dispensable for cellular proliferation. (t-test, n = 4) Scale bars = 50µm.