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. 2018 May 31;14(5):e1007040. doi: 10.1371/journal.ppat.1007040

Fig 5. Characterisation of cells infected by MCMV within the iris.

Fig 5

(A) Wholemounts of the iris were prepared at 24 hr pi and stained with antibodies to CD31 (red) and the IE1 viral antigen (green). Iris whole-mounts at day 2 pi were stained with anti-IE1 (green) and (B) anti-CD31 or (C) anti-PDGFRβ antibodies. Blue = DAPI nuclear stain. (D) Wholemounts of the iris at day 5 pi were stained with anti-IE1 (green) along with antibodies directed against the indicated cell surface markers (red). Anti-IE1 detects a nuclear antigen. Scale bar: 50 μm. Results are representative of at least 3 independent experiments with n≥9.