Table 8. Hemolytic activity against horse erythrocytes, calculated hydrophobicity, hydrophobic moment and net charge of Cap18 and Cap18 derivatives.

Peptide	Substitution	Peptide concentration [μg/ml]	Haemolytic Activity [%]	Hydrophobicity <H>	Hydrophobic Moment <μH>	Net Charge
Cap18 –pure1)	original	64	1% ± 1%	0.107	0.471	+12
Cap18 –library2)	original	32	2% ± 1%	0.107	0.471	+12
Peptide 1	L6P	64	1%	0.080	0.445	+12
Peptide 2	R10L	64	6%	0.180	0.518	+11
Peptide 3	I13D	64	1%	0.037	0.402	+11
Peptide 4	I13F	64	2%	0.106	0.471	+12
Peptide 5	I13H	64	1%	0.062	0.426	+12
Peptide 6	I13M	64	2%	0.091	0.456	+12
Peptide 7	I13Q	64	1%	0.052	0.417	+12
Peptide 8	I13S	64	1%	0.057	0.421	+12
Peptide 9	K16C	64	4%	0.175	0.502	+11
Peptide 10	K16D	64	1%	0.113	0.473	+10
Peptide 11	K16F	64	9%	0.182	0.505	+11
Peptide 12	K16I	64	10%	0.182	0.505	+11
Peptide 13	K16L	64	14%	0.179	0.504	+11
Peptide 14	K16M	64	8%	0.167	0.497	+11
Peptide 15	K16Y	64	9%	0.159	0.494	+11
Peptide 16	L17D	64	0%	0.040	0.417	+11
Peptide 17	L17K	64	1%	0.034	0.412	+13
Peptide 18	L17P	64	0%	0.080	0.449	+12
Peptide 19	K18P	64	1%	0.153	0.441	+11
Peptide 20	I20E	64	1%	0.041	0.413	+11
Peptide 21	I20H	64	1%	0.062	0.431	+12
Peptide 22	I20N	64	0%	0.042	0.414	+12
Peptide 23	G21C	64	4%	0.148	0.485	+12
Peptide 24	G21L	64	7%	0.153	0.486	+12
Peptide 25	I24C	64	2%	0.100	0.464	+12
Peptide 26	I24D	64	0%	0.037	0.403	+11
Peptide 27	I24G	64	1%	0.058	0.424	+12
Peptide 28	I24N	64	1%	0.042	0.408	+12
Peptide 29	I24S	64	1%	0.057	0.423	+12
Peptide 30	G26T	64	2%	0.114	0.465	+12
Peptide 31	L27P	64	1%	0.080	0.453	+12
Peptide 32	P29A	64	4%	0.096	0.481	+12
Peptide 33	P29D	64	3%	0.066	0.507	+11
Peptide 34	P29F	64	7%	0.136	0.445	+12
Peptide 35	P29H	64	3%	0.091	0.485	+12
Peptide 36	P29S	64	4%	0.086	0.489	+12

¹⁾ Purity ≥89.5%

²⁾ Purity 47.5%

The hemolytic activity is measured in duplicates and given as the average ± SD in % relative to full lysis by 0.2% triton X-100. Mean hydrophobicity <H>, hydrophobic moment <μH>, and net charge are calculated using heliquest (http://heliquest.ipmc.cnrs.fr).