Figure 10.

Effects of SR7826 and LIMKi3 on phosphorylations in WPMY‐1 cells. WPMY‐1 cells were incubated with SR7826 (5 μM, 2 h), LIMKi3 (5 μM, 2 h) or DMSO (control, 2 h). Subsequently, the phosphorylation states of cofilin, LIMK and MYPT1 (threonine 696), MLC and 4E‐BP1 (threonine 37/46, serine 65) were semiquantitatively compared between inhibitor and control groups by Western blot analyses using site‐ and phospho‐specific antibodies; values for each sample were normalized to the mean of the corresponding control group. Shown are representative blots, and means ± SEM from a series of five independent experiments (^* P < 0.05 for control vs. inhibitor).