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. 2018 May 18;14(5):e1007074. doi: 10.1371/journal.ppat.1007074

Fig 2. pilW, pilX, and pilY1 mutants have reduced cdrA promoter activity.

Fig 2

(A) cdrA promoter activity in PA14 sadC and algR deletion and overexpression strains. pMS402-PcdrA, containing the lux genes under expression of the cdrA promoter, was introduced into strains of interest, along with pBADGr (vector-only control), pBADGr-sadC, or pBADGr-algR. Assays were set up in technical triplicate in SK media, with or without 0.05% L-arabinose to induce expression of the pBADGr promoter, and measurements were taken every 15 min over 5 h. Loss of sadC or algR led to a subtle decrease in cdrA promoter activity, while SadC overexpression led to a dramatic increase in cdrA promoter activity. Overexpression of AlgR also led to a subtle increase in cdrA promoter activity that was enhanced upon addition of L-arabinose. n = 3 trials. (B) cdrA promoter activity in PA14 pilA, fimU, pilV, pilW, pilX, pilY1, and pilE mutants. Loss of pilW, pilX, or pilY1 led to a decrease in cdrA promoter activity. n = 3 trials.