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SK assays for PA14 pilW, pilX, pilY1, algU, pilW algU, pilX algU, and pilY1 algU mutants. Loss of algU led to more rapid killing relative to WT, while pilW algU, pilX algU, and pilY1 algU mutants had near-WT virulence. Asterisks indicate strains that were significantly different from PA14 by Gehan-Breslow-Wilcoxon test at p = 0.05 (p = 0.00625 with a Bonferroni correction), n = 3.
