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. Author manuscript; available in PMC: 2018 May 31.
Published in final edited form as: Nat Chem Biol. 2018 Feb 14;14(3):188–192. doi: 10.1038/nchembio.2572

Figure 2.

Figure 2

Basic applications of modified recombinant proteins. (a) Assay to assess the effect of a PTM on enzyme activity. Kinetic comparisons of unmodified and various modified forms of the enzyme can be performed to elucidate the effect of PTMs at specific positions. (b) Identification of PTM-dependent interactors, or development of new PTM biosensors. Modified proteins can be used as scaffolds for identifying or evolving new PTM-dependent binding interactions. (c) Drug screens to identify inhibitors of PTM-activated proteins. Modified proteins are important reagents to identify candidate small molecule inhibitors that specifically target only modified forms of a protein of interest. Modified proteins are often implicated in disease and can serve as excellent therapeutic targets.