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. 2018 Apr 4;19(4):1079. doi: 10.3390/ijms19041079

Figure 5.

Figure 5

Oral delivery of dsRNAs. (A) Length and width of A. pisum 10 days after feeding on dsRNA containing diets. Asterisks depict significant differences **** p < 0.0001 and ** p < 0.01 relative to control treatments; (B) Survival of T. castaneum after feeding 1st–2nd instar larvae on flour discs containing Tc-VTE and Tc-th dsRNAs (n = 20 per treatment) for 30 days. Asterisks depict significant differences **** p < 0.0001 and ** p < 0.002 relative to control treatments; (C) Relative expression of Tc-VTE and Tc-th mRNAs in T. castaneum larvae after 10 days after feeding on flour discs containing dsRNAs (500 ng/mg diet). Error bars indicate the ±SD of the mean from three independent biological replicates (n = 5 insects per treatment), each with three technical replicates. RQ set to 1.0 for nptII control 10-day biological replicate 1. Asterisks depict significant differences **** p < 0.0001 and ** p = 0.0047 relative to control treatments.