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. 2018 Apr 4;19(4):1079. doi: 10.3390/ijms19041079

Table 1.

Sequence of forward (F)/reverse (R) primers used for cDNA sub-cloning, dsRNA synthesis and qPCR analysis. Tc denotes T. castaneum and Ap denotes A. pisum.

Gene (Accession No.) Insect Species Sequence 5′-3′
V-type ATPase E subunit
(XM_965528)		 Tc cDNA sub-cloning and in vitro transcription:
F: TATCTCGAGACCAGGCGAGATATTCACAGC
R: TATCTCGAGAAACGAGCCTCCAAGGTGTTG
qPCR analyses:
F: CCAAGCATTTTTAATGCA CCAC
R: AACCACCACGACCTTGAATAG
Inhibitor of Apoptosis
(XM_969968.2)		 Tc cDNA sub-cloning and in vitro transcription:
F: ATATCTAGAAGTTCGGCTGTAACTCCCG
R: ATACTAGACATCCGGAACGTCTCACTCT
qPCR analyses:
F: AAGCGAAAAGTTGAGGCAAGC
R: AACCATTGCTTTCTTACTCGAAGG
GAPDH
(XP_974181.1)		 Tc qPCR analyses:
F: CCGGGATGGCGTTCAG
R: CCAAACGCACCGTCAAATC
V-type ATPase E subunit
(XM_001946489)		 Ap cDNA sub-cloning and in vitro transcription:
F: TATCTCGAGGGGCCGCCTGGT
R: ATATCTAGACACGAACACGTAATGTGA
qPCR analyses:
F: CCGAGTATAAGGCAGCATCCA
R: CTTATGTGCCAACAACTCAATACCA
Inhibitor of Apoptosis
(XM_001944122.3)		 Ap cDNA sub-cloning and in vitro transcription:
F: TATCTCGAGGGTCTGAAGGACTGGGAAGAA
R: GCTTCCGGCGTAGGTGTTCTAGAATA
qPCR analyses:
F: GATTATTGGCAACAAGGTGATGATC
R: AACCAGCAGAAGAATCGTTAAAAAA
GAPDH
(NM_001293474.1)		 Ap qPCR analyses:
F: CAATGGAAACAAGATCAAGGTGTT
R: ACCAGCAGATCCCCATTGG
Kanamycin resistance
(JN638547)		 - F: AGGCTATTCGGCTATGAC
R: CGATAGAAGGCGATGCG