Skip to main content
. 2018 Apr 18;19(4):1233. doi: 10.3390/ijms19041233

Figure 3.

Figure 3

The miR-26a-5p-mediated regulation of sperm quality. (A) Relative expression levels of miR-26a-5p in control, mimic- and inhibitor-transfected sperm. (B) Effect of miR-26a-5p mimic and inhibitor on three velocity parameters of sperm cells, including curvilinear velocity (VCL, μm/s), average path velocity (VAP, μm/s) and straight line velocity (VSL, μm/s). (C) Effect of miR-26a-5p mimics and inhibitor on sperm motility rate (%). No significant changes were observed in (D) amplitude of lateral head displacement (ALH, μm), (E) sperm density (million/mL), (F) motility parameter wobble (WOB, %), or (G) linearity (LIN, %) and STR (%). (H) Effect of miR-26a-5p mimics and inhibitor on the expression levels of apoptosis-related genes. Con, control; Mimi, mimics; MC, mimic control; Inhi, inhibitor; IC, inhibitor control. CT and HC represent prepubertally hemicastrated Yorkshire boars and normal controls, respectively. Three independent experiments were performed in triplicate and all data are expressed as means ± SD. * p < 0.05, ** p < 0.01.